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Objective To explore the curative effect of combination therapy of low-temperature plasma radiofrequency ablation and nasal endoscopy for chronic nasal sinusitis. Methods From January 2010 to January 2012,98 patients with chronic nasal sinusitis in our hospital were ramdomly divided into observation group and control group. The observation group (49 cases) received plasma radiofrequency ablation at low temperature combined nasal endoscopic treatment. The control group (49 cases) were performed only nasal endoscopic treatment. The curative effect of the 2 groups were compared. Results After 1 month and 1 year, the total effective rate of the observation group were 91. 84% and 89. 80%, the total effective rate of the control group were 77. 55% and 73. 47% respectively. To compare the total effective rate of 2 groups were compared,there were statistically significant differences(P<0. 05). Conclusion Curative effect of plasma radiofrequency ablation at low temperature and nasal endoscopy is distinct,it is worthy of being widely applied in clinic.
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OBJECTIVE@#To probe into both the quality of life and personality characteristics of allergic rhinitis (AR) patients.@*METHOD@#AR patients were investigated by questionnaires of SRF-36, EPQ, SAS, SDS, then the influencing factors were analyzed by multiple regression analysis.@*RESULT@#People suffered from AR were influenced on quality of life, especially on role-physical, general health, role-emotional; there was no obviously difference on personality characteristics; however, anxiety tendency but no depressive emotion was observed.@*CONCLUSION@#In order to seek better treatment under new medical model, we should put emphasis on the quality of life and personality characteristics of AR patients.
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Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Asthma , Epidemiology , Psychology , Quality of Life , Rhinitis, Allergic, Perennial , Epidemiology , Psychology , Surveys and QuestionnairesABSTRACT
OBJECTIVE@#To study the inhibitory effects of short hairpin RNA (pshLMP1) combined with DNAzyme or alone on latent membrane protein-1 (LMP1)expression in HNE1 cell lines.@*METHOD@#The pshLMP1 and DNAzymes were co-transfected with pEGFP-N1-1158 (a expression vector encoding LMP1 and EGFP fusion protein) into HNE1 cells, divided into 4 groups as positive control, RNA interference, combining and DNAzyme groups. The green fluorescence were analyzed and then the mRNA and protein of LMP1 gene were detected.@*RESULT@#The HNE1 cells expressing green fluorescence in combining group were significantly less than that in RNA interference group (P < 0.01); The inhibition efficiency of green fluorescence in combining group were 86. 31% Wp 88.88% ,which were higher than that in RNA interference group with 75.15%. The detection of LMP1 mRNA and proteins showed that combining group had a higher inhibition ability.@*CONCLUSION@#Combining gene therapy with pshLMP1 and DNAzyme is superior to pshLMP1 alone to inhibit LMP1 gene expression.
Subject(s)
Humans , Cell Line, Tumor , DNA, Catalytic , Therapeutic Uses , Exons , Gene Expression Regulation, Viral , Genetic Therapy , Nasopharyngeal Neoplasms , Genetics , Metabolism , Pathology , RNA, Small Interfering , Genetics , Therapeutic Uses , Transfection , Viral Matrix Proteins , GeneticsABSTRACT
OBJECTIVE@#To detect and analyze the EBV-LMP1 gene N terminus XhoI-site mutation and C terminus 30 bp deletion in plasma of patients with nasopharyngeal carcinoma (NPC) in Chongqing of China.@*METHOD@#DNA extraction and PCR amplification was used in plasma of 48 NPC patients and 40 control non-NPC cases from Chongqing of China. All the PCR production of N terminus was digested by enzyme XhoI, then segregated in 8% PAGE. All- the PCR production of C terminus was segregated in 8% PAGE too. Compared with B95-8 cell, some of the PCR production were sequenced and analyzed with software.@*RESULT@#LMP1 was amplified successfully from 48 of 48 NPC cases (100%) and from 38 of 40 non-NPC cases (95%) by PCR, but none of LMP1 XhoI-site mutation and none of 30 bp deletion was found by digesting, segregating and Sequencing.@*CONCLUSION@#EBV-LMP1 gene N terminus XhoI-site and C terminus 30 bp deletion have no mutation in plasma of NPC or non-NPC cases in Chongqing of China. The precise relationship of EBV-LMP1 gene mutation or deletion with NPC pathogenesis needs further investigation.
Subject(s)
Adult , Female , Humans , Male , Middle Aged , China , DNA, Viral , Genetics , Herpesvirus 4, Human , Nasopharyngeal Neoplasms , Blood , Genetics , Pathology , Virology , Sequence Deletion , Viral Matrix Proteins , Blood , GeneticsABSTRACT
On the basis of experiences in advanced otolaryngology-head & neck surgeons teaching,we realized that it was important to accomplish the following measures:to insist on entering instruction,to make reasonable training plan,to hold regular lecture,to attach importance to applied anatomy teaching,to cultivate clinical ability,to standardize medical record writing,to strengthen communication between doctors and patients and to strengthen medical morality construction.Only by doing so can we improve the advanced education quality.
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The application and expanding of nasal endoscopic surgery,an epoch-making transform of rhinology,have a very close associations with medical imaging,which includes anatomy photograph,conceptual diagram,radiological imaging,nasal endoscopic image display system,operation photographic recording and computer assisted instruction courseware,etc.It is important to collect the medical imaging through various means and set up medical imaging resources library for nasal endoscopic surgery teaching.
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Objectives:To determine the common allergens of patients with allergic rhinitis in Chongqing.Methods:A total of 738 cases who were diagnosed as allergic rhinitis by history and clinical presentation,underwent a skin prick test with 11 standardized aeroallergens and 10 ingestive allergens,and the important sensitizing allergens were assessed.Difference of skin prick test responses at various ages and with different gender was investigated.Results:The percent of patients with positive skin tests in inhalation allergens were as follows,dust(91.33%),acaridan(83.88%),prevemal pollen(68.97%),late spring pollen (66.67%),estivoautumnal pollen (61.25%),tobacco (58.94%) and cockroach (55.28%).Allergens in food were mainly prawn (62.47%) and crab(60.43%).Conclusion:The study shows that the dust,acaridan,prevemal pollen,late spring pollen and estivoautumnal pollen are the most important allergens in Chongqing region.